Article
NsrR-dependent method for detecting nitric oxide accumulation in the Escherichia coli cytoplasm and enzymes involved in NO production.
FEMS microbiology letters - 1 Dec 2011
Vine Claire E, Purewal Sukhjit K, Cole Jeffrey A
Abstract excerpt
A β-galactosidase assay for detecting the accumulation of NO in the Escherichia coli cytoplasm has been developed based on the sensitive response of the transcription repressor, NsrR, to NO. The hcp promoter is repressed by NsrR in the absence of nitric oxide, but repression is relieved when NO accumulates in the cytoplasm. Most, but not all, of this NO is formed by the interaction of the membrane-associated...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
