Article
Heterologous NNR-mediated nitric oxide signaling in Escherichia coli.
Journal of bacteriology - 1 Nov 2000
Hutchings M I, Shearer N, Wastell S, van Spanning R J, Spiro S
Abstract excerpt
The transcription factor NNR from Paracoccus denitrificans was expressed in a strain of Escherichia coli carrying a plasmid-borne fusion of the melR promoter to lacZ, with a consensus FNR-binding site 41.5 bp upstream of the transcription start site. This promoter was activated by NNR under anaerobic growth conditions in media containing nitrate, nitrite, or the NO(+) donor sodium nitroprusside. Activation by...
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