Article
Evaluation of a Multiplex Real-Time Polymerase Chain Reaction for the Quantification of <i>Escherichia coli</i> O157 in Cattle Feces
2 Nov 2011
Abstract excerpt
Cattle are asymptomatic reservoirs for Escherichia coli O157, a major foodborne pathogen. The organism generally colonizes the hindgut of cattle and is shed in the feces at low concentrations. The objective of this research was to evaluate a multiplex, real-time polymerase chain reaction (mqPCR) assay for quantification of E. coli O157 in cattle feces using stx1, stx2, and rfbE gene targets. Primer efficiency and...
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