Article
Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons.
BMC microbiology - 2 Nov 2011
Siddiqui Huma, Nederbragt Alexander J, Lagesen Karin, Jeansson Stig L, Jakobsen Kjetill S
Abstract excerpt
BACKGROUND: Urine within the urinary tract is commonly regarded as "sterile" in cultivation terms. Here, we present a comprehensive in-depth study of bacterial 16S rDNA sequences associated with urine from healthy females by means of culture-independent high-throughput sequencing techniques. RESULTS: Sequencing of the V1V2 and V6 regions of the 16S ribosomal RNA gene using the 454 GS FLX system was performed to...
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