Article
Gene-enzyme relationship in the sulfate assimilation pathway of Saccharomyces cerevisiae. Study of the 3'-phosphoadenylylsulfate reductase structural gene.
The Journal of biological chemistry - 15 Sept 1990
Thomas D, Barbey R, Surdin-Kerjan Y
Abstract excerpt
In yeast, mutations in six different loci (MET1, MET4, MET8, MET16, MET22, and MET25) have been reported to result in the absence of 3'-phosphoadenylylsulfate (PAPS) reductase activity. In the present study, we show that MET16 is the structural gene for PAPS reductase and that the yeast and the Escherichia coli enzymes display significant similarities. Thioredoxin has been implicated in the reduction of PAPS in...
Topics
- Amino Acid Sequence
- Base Sequence
- Escherichia coli
- Genes, Fungal
- Genetic Vectors
- Genotype
- Molecular Sequence Data
- Oxidoreductases
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae