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Article

High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type".

Journal of microbiological methods - 1 Jan 2012

Douarre Pierre E, Cashman William, Buckley Jim, Coffey Aidan, O'Mahony Jim M

Abstract excerpt

In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.

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Source

Identifiers

PMID: 22033498
DOI: 10.1016/j.mimet.2011.10.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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