Article
High-level expression of the FtsA protein inhibits cell septation in Escherichia coli K-12.
Journal of bacteriology - 1 Aug 1990
Wang H C, Gayda R C
Abstract excerpt
DNA fragments encoding the ftsA gene were subcloned into plasmids downstream of a lac promoter or a tac promoter. These plasmid constructs, when transformed into wild-type and mutant strains, inhibited normal cell septation, causing the formation of long nonseptate filaments. This phenotype is due to overproduction of the FtsA protein.
Topics
- Bacterial Proteins
- Cell Division
- Cloning, Molecular
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression
- Genes, Bacterial
- Isopropyl Thiogalactoside
- Mutation
- Plasmids
- Restriction Mapping