Article
[The transformation of legionellae by plasmid DNA].
Zhurnal mikrobiologii, epidemiologii i immunobiologii - 1 Mar 1990
Marakusha B I, Feoktistova E Iu, Tartakovskiĭ I S, Prozorovskiĭ S V
Abstract excerpt
For the first time the possibility of the genetic transformation of L. pneumophila and L. bozemanii strains with the use of purified DNA of plasmids pUC19, pUC4K, pSC101 and RSF1010-pBR322 was shown. The frequency of transformation varied from 5.2 x 10(-6) to 5.8 x 10(-7), depending on the strain used in the experiment and plasmid DNA. In some of the transformants obtained in this investigation plasmid DNA whose...
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