Article
[Construction of the gene library of symbiotic nitrogen-fixing Rhizobium lupini].
Prikladnaia biokhimiia i mikrobiologiia - 1 Jan 2000
Ivanushkin A G, Marchenko G N, Chistoserdov A Iu, Pushkin A V, Kretovich V L
Abstract excerpt
The gene bank of the symbiotic nitrogen-fixing bacterium Rhizobium lupini (effective strain 359a) was constructed on plasmid pAYC31 that was used to transform Escherichia coli C6000. The bank contains 6600 clones. Restriction analysis showed that the size of the mean insertion fragment in the plasmid in 6.5 kb.
Topics
- Escherichia coli
- Gene Library
- Genes, Bacterial
- Mutation
- Nitrogen Fixation
- Plasmids
- Rhizobium