Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic diversity of Mycobacterium tuberculosis in Delhi, North India.

doi:10.1590/s0074-02762011000500002

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Article

Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic diversity of Mycobacterium tuberculosis in Delhi, North India.

Memorias do Instituto Oswaldo Cruz - 1 Aug 2011

Varma-Basil Mandira, Kumar Sujeet, Arora Jyoti, Angrup Archana, Zozio Thierry, Banavaliker Jayant Nagesh, Singh Urvashi Balbir, Rastogi Nalin, Bose Mridula

Abstract excerpt

The aim of the present study was to compare polymerase chain reaction (PCR)-based methods--spoligotyping and mycobacterial interspersed repetitive units (MIRU) typing--with the gold-standard IS6110 restriction fragment length polymorphism (RFLP) analysis in 101 isolates of Mycobacterium tuberculosis to determine the genetic diversity of M. tuberculosis clinical isolates from Delhi, North India. Spoligotyping...

Topics

Adult
Bacterial Typing Techniques
Cluster Analysis
DNA, Bacterial
Female
Genetic Variation
Genotype
Humans
India
Male

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Minisatellite Repeats

Mycobacterium tuberculosis

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Repetitive Sequences, Nucleic Acid

Young Adult

Source

Open in PubMed

Identifiers

PMID: 21894371
DOI: 10.1590/s0074-02762011000500002

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