Article
Visual detection of high-risk human papillomavirus genotypes 16, 18, 45, 52, and 58 by loop-mediated isothermal amplification with hydroxynaphthol blue dye.
Journal of clinical microbiology - 1 Oct 2011
Luo Le, Nie Kai, Yang Meng-Jie, Wang Miao, Li Jin, Zhang Chen, Liu Hong-Tu, Ma Xue-Jun
Abstract excerpt
A simple, rapid, sensitive, qualitative, colorimetric loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye (HNB) was established to detect high-risk human papillomavirus (HPV) genotypes 16, 18, 45, 52, and 58. All initial validation studies with the control DNA proved to be type specific. The colorimetric type-specific LAMP assay could achieve a sensitivity of 10 to 100 copies at 63°C for...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.