Article
[Colorimetric detection of HPV6 and HPV16 by loop mediated isothermal amplification].
Bing du xue bao = Chinese journal of virology - 1 Jan 2011
Lu Chun-bin, Luo Le, Yang Meng-jie, Nie Kai, Wang Miao, Ma Xue-Jun
Abstract excerpt
A simple, rapid and sensitive colorimetric loop mediated isothermal amplification (LAMP) method was established to detect HPV6 and HPV 16 respectively. The method employed a set of four specially designed primers that recognized six distinct sequences of HPV6-E6 or HPV16-E7 for amplification of nucleic acid under isothermal conditions at 63 degrees C for one hour. The amplification process of LAMP was monitored...
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