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Article

Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3).

PloS one - 1 Jan 2011

Pinske Constanze, Bönn Markus, Krüger Sara, Lindenstrauss Ute, Sawers R Gary

Abstract excerpt

The Escherichia coli B strain BL21(DE3) has had a profound impact on biotechnology through its use in the production of recombinant proteins. Little is understood, however, regarding the physiology of this important E. coli strain. We show here that BL21(DE3) totally lacks activity of the four [NiFe]-hydrogenases, the three molybdenum- and selenium-containing formate dehydrogenases and molybdenum-dependent...

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Identifiers

PMID: 21826210
DOI: 10.1371/journal.pone.0022830
PMCID: PMC3149613

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Source receipt: pubmed, retrieved 7 Sept 2026.

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