Article
Genetic analysis of rec E activities in Bacillus subtilis.
Molecular & general genetics : MGG - 1 Jul 1990
Ceglowski P, Lüder G, Alonso J C
Abstract excerpt
A recE mutant (recE6) of Bacillus subtilis was constructed by insertion of a selectable marker into the recE coding region. The insertional inactivation of the recE gene renders cells very sensitive to DNA damaging agents and severely impairs intermolecular recombination, but does not markedly af...
Topics
- 4-Nitroquinoline-1-oxide
- Alleles
- Bacillus subtilis
- Cloning, Molecular
- DNA Damage
- DNA Repair
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli Proteins
- Exodeoxyribonucleases
- Exons
- Genes, Bacterial
- Genetic Markers
- Mutation
- Recombination, Genetic
- Transduction, Genetic
- Transfection
- Transformation, Bacterial
