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Article

Genetic analysis of fusion recombinants in Bacillus subtilis: function of the recE gene.

Genetics - 1 Nov 1990

Ftouhi N, Guillén N

Abstract excerpt

Bacillus subtilis protoplast fusion allows the study of the genetic recombination of an entire procaryotic genome. Protoplasts from bacterial strains marked genetically by chromosomal mutations were fused using polyethylene glycol and the regenerated cells analyzed. Recombinants represent 19.3% of heterozygotic cells; they are haploids. Individual characterization of clones show a unique particular phenotype in...

Topics

Bacillus subtilis
Blotting, Southern
Chromosomes, Bacterial
Diploidy
Escherichia coli Proteins
Exodeoxyribonucleases
Genes, Bacterial
Heterozygote
Mutation
Phenotype
Polyethylene Glycols

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombination, Genetic

Restriction Mapping

Transformation, Bacterial

Source

Open in PubMed Open full text

Identifiers

PMID: 2123461
DOI: 10.1093/genetics/126.3.487
PMCID: PMC1204206

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2123461-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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