Article
Direct genotyping of Toxoplasma gondii from amniotic fluids based on B1 gene polymorphism using minisequencing analysis.
BMC infectious diseases - 19 Nov 2013
Costa Jean-Marc, Alanio Alexandre, Moukoury Sandrine, Clairet Vincent, Debruyne Monique, Poveda Jean-Dominique, Bretagne Stéphane
Abstract excerpt
BACKGROUND: Because some Toxoplasma gondii genotypes may be more virulent in pregnant women, discriminating between them appears valuable. Currently, the main genotyping method is based on single copy microsatellite markers, which limit direct genotyping from amniotic fluids (AFs) to samples with a high parasitic load. We investigated whether the multicopy gene B1 could type the parasite with a higher...
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