Article
Loss of Tdn catabolic genes by deletion from and curing of plasmid pTDN1 in Pseudomonas putida: rate and mode of loss are substrate and pH dependent.
Journal of general microbiology - 1 Apr 1990
Saint C P, Venables W A
Abstract excerpt
The ability to degrade aromatic amines and m-toluate (Tdn+ phenotype), encoded by plasmid pTDN1, was lost from Pseudomonas putida hosts after subculture in benzoate, succinate, acetate and glucose minimal medium, the fastest rate of loss occurring where benzoate was the substrate. Tdn- cells had either lost the entire pTDN1 plasmid or suffered a recombinational deletion of a specific 26 kbp region. Proportional...
Topics
- Aniline Compounds
- Benzoates
- Benzoic Acid
- Chromosome Deletion
- DNA Transposable Elements
- Hydrogen-Ion Concentration
- Phenotype
- Plasmids
- Pseudomonas
- Toluidines