Article
Isolation of behavioral mutants of Azospirillum brasilense by using Tn5 lacZ.
Applied and environmental microbiology - 1 Apr 1990
van Rhijn P, Vanstockem M, Vanderleyden J, De Mot R
Abstract excerpt
Tn5 lacZ mutants were generated with Azospirillum brasilense 7030 by mating that strain with Escherichia coli strains carrying suicide plasmid pCIB100 or pCIB110. Kanamycin-resistant Azospirillum colonies were obtained with a maximum frequency of 10(-6) per recipient cell. The potential of Tn5 lacZ for random transposon mutagenesis coupled to transcription analysis in A. brasilense 7030 was demonstrated. Sixty...
Topics
- Azospirillum brasilense
- Cell Movement
- Chemotaxis
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Lac Operon
- Molecular Weight
- Mutation
- Plasmids