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Article

Isolation of behavioral mutants of Azospirillum brasilense by using Tn5 lacZ.

Applied and environmental microbiology - 1 Apr 1990

van Rhijn P, Vanstockem M, Vanderleyden J, De Mot R

Abstract excerpt

Tn5 lacZ mutants were generated with Azospirillum brasilense 7030 by mating that strain with Escherichia coli strains carrying suicide plasmid pCIB100 or pCIB110. Kanamycin-resistant Azospirillum colonies were obtained with a maximum frequency of 10(-6) per recipient cell. The potential of Tn5 lacZ for random transposon mutagenesis coupled to transcription analysis in A. brasilense 7030 was demonstrated. Sixty...

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Identifiers

PMID: 2160221
DOI: 10.1128/aem.56.4.990-996.1990
PMCID: PMC184333

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