Article
Expression of Bacillus thuringiensis delta-endotoxin genes during vegetative growth.
Applied and environmental microbiology - 1 Apr 1990
Mettus A M, Macaluso A
Abstract excerpt
Bacillus thuringiensis delta-endotoxin (crystal protein) genes are normally expressed only during sporulation. It is possible to produce crystal protein during vegetative growth by placing B. thuringiensis crystal protein genes downstream of a strong vegetative promoter. By removing a possible transcriptional terminator of the tetracycline resistance gene of pBC16 and inserting a multiple cloning site,...
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