Article
Temporal and spatial regulation of the symbiotic genes of Rhizobium meliloti in planta revealed by transposon Tn5-gusA.
Genes & development - 1 Mar 1990
Sharma S B, Signer E R
Abstract excerpt
Tn5-gusA promoter/probe transposons have been constructed that fuse the Escherichia coli gusA reporter gene transcriptionally or translationally with a target promoter. These have been used to monitor expression of Rhizobium meliloti symbiotic genes within alfalfa nodules. Fusions in all 11 nod genes studied show the same pattern of expression: first on the root surface, then throughout the developing nodule,...
Topics
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Molecular Sequence Data
- Phenotype
- Promoter Regions, Genetic
- Restriction Mapping