Article
Heterologous expression of the Oceanobacillus iheyensis SigW and its anti-protein RsiW in Bacillus subtilis.
Bioscience, biotechnology, and biochemistry - 1 Jan 2011
Yano Koichi, Inoue Hiromi, Mori Hirokazu, Yee Lii Mien, Matsuoka Satoshi, Sadaie Yoshito, Asai Kei
Abstract excerpt
Pairs of the ECF sigma factor and its anti-sigma factor, SigW and RsiW, of Bacillus-related species that inhabit extreme environments were heterologously expressed in B. subtilis. All the RsiWs, membrane proteins, failed to fill their function of repressing cognate SigW activity, despite their close structural similarities. Particularly, uncontrolled expression of Oceanobacillus iheyensis OISigW due to abortive...
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