Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications.

Abstract excerpt

Two anthranilate synthase gene pairs have been identified in Pseudomonas aeruginosa. They were cloned, sequenced, inactivated in vitro by insertion of an antibiotic resistance gene, and returned to P. aeruginosa, replacing the wild-type gene. One anthranilate synthase enzyme participates in tryptophan synthesis; its genes are designated trpE and trpG. The other anthranilate synthase enzyme, encoded by phnA and...

Topics

• Amino Acid Sequence
• Anthranilate Synthase
• Base Sequence
• Biological Evolution
• Blotting, Southern
• Cloning, Molecular
• DNA Transposable Elements
• DNA, Bacterial
• Escherichia coli
• Genes, Bacterial