Article
Cysteine shotgun-mass spectrometry (CS-MS) reveals dynamic sequence of protein structure changes within mutant and stressed cells.
Proceedings of the National Academy of Sciences of the United States of America - 17 May 2011
Krieger Christine C, An Xiuli, Tang Hsin-Yao, Mohandas Narla, Speicher David W, Discher Dennis E
Abstract excerpt
Questions of if and when protein structures change within cells pervade biology and include questions of how the cytoskeleton sustains stresses on cells--particularly in mutant versus normal cells. Cysteine shotgun labeling with fluorophores is analyzed here with mass spectrometry of the spectrin-actin membrane skeleton in sheared red blood cell ghosts from normal and diseased mice. Sheared samples are compared...
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