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Cytochrome b Gene Quantitative PCR for Diagnosing Plasmodium falciparum Infection in Travelers

21 Apr 2011

Abstract excerpt

A cytochrome b (cytb) gene quantitative PCR (qPCR) assay was developed to diagnose malaria in travelers. First, manual and automated DNA extractions were compared and automated DNA extraction of 400 μl of blood was found to be more efficient. Sensitivity was estimated using the WHO international standard for Plasmodium falciparum DNA and compared to that of a previously published qPCR targeting the 18S rRNA...

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PMID: 21508150
DOI: 10.1128/jcm.02156-10

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