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Assessment of malaria real-time PCR methods and application with focus on low-level parasitaemia

5 Jul 2019

Abstract excerpt

In epidemiological surveys and surveillance the application of molecular tools is essential in detecting submicroscopic malaria. A genus-specific conventional cytochrome b (cytb) PCR has shown high sensitivity in field studies, detecting 70% submicroscopic malaria. The main objective of this study was to assess the conversion from conventional to real-time PCR testing both SYBR and probe protocols, and including...

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PMID: 31276473
DOI: 10.1371/journal.pone.0218982

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