Article
Virulence of Pasteurella multocida recA mutants.
Veterinary microbiology - 3 May 2001
Cárdenas M, Fernández de Henestrosa A R, Campoy S, Perez de Rozas A M, Barbé J, Badiola I, Llagostera M
Abstract excerpt
In order to determine the role of the RecA protein in the virulence of Pasteurella multocida, a recA mutant was constructed and used in studies of virulence and competition in relation to wild-type strain. To achieve this, firstly, the recA gene was isolated and sequenced, showing an Escherichia coli-like SOS box and encoding a protein of 354 amino acids which has the closest identity with the Haemophilus...
Topics
- Animals
- Blotting, Southern
- DNA Repair
- DNA, Bacterial
- Lethal Dose 50
- Mice
- Mutation
- Pasteurella multocida
- Rec A Recombinases