Article
Expression of RNA-interference/antisense transgenes by the cognate promoters of target genes is a better gene-silencing strategy to study gene functions in rice.
PloS one - 3 Mar 2011
Li Jing, Jiang Dagang, Zhou Hai, Li Feng, Yang Jiawei, Hong Laifa, Fu Xiao, Li Zhibin, Liu Zhenlan, Li Jianming, Zhuang Chuxiong
Abstract excerpt
Antisense and RNA interference (RNAi)-mediated gene silencing systems are powerful reverse genetic methods for studying gene function. Most RNAi and antisense experiments used constitutive promoters to drive the expression of RNAi/antisense transgenes; however, several reports showed that constitutive promoters were not expressed in all cell types in cereal plants, suggesting that the constitutive promoter...
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