Duplication-induced mutation of a new Neurospora gene required for acetate utilization: properties of the mutant and predicted amino acid sequence of the protein product.

Abstract excerpt

A cloned Neurospora crassa genomic sequence, selected as preferentially transcribed when acetate was the sole carbon source, was introduced in extra copies at ectopic loci by transformation. Sexual crossing of transformants yielded acetate nonutilizing mutants with methylation and restriction site changes within both the ectopic DNA and the normally located gene. Such changes are typical of the...

Topics

• Acetates
• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• Codon
• Genes, Fungal
• Kinetics
• Molecular Sequence Data
• Mutation
• Neurospora
• Neurospora crassa