Article
Overexpression, purification, and characterization of isochorismate synthase (EntC), the first enzyme involved in the biosynthesis of enterobactin from chorismate.
Biochemistry - 13 Feb 1990
Liu J, Quinn N, Berchtold G A, Walsh C T
Abstract excerpt
Isochorismate synthase (EC 5.4.99.6), the entC gene product of Escherichia coli, catalyzes the conversion of chorismate to isochorismate, the first step in the biosynthesis of the powerful iron-chelating agent enterobactin. A sequence-specific deletion method has been used to construct an EntC overproducer, which allows for the purification and characterization of the E. coli isochorismate synthase for the first...
Topics
- Base Sequence
- Chorismic Acid
- Cloning, Molecular
- Cyclohexanecarboxylic Acids
- Enterobactin
- Gene Expression Regulation, Enzymologic
- Intramolecular Transferases
- Isomerases
- Kinetics
- Magnetic Resonance Spectroscopy