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Article

Isolation and characterization of two new hepatitis E virus genotype 1 strains from two mini-outbreaks in Lahore, Pakistan.

Virology journal - 4 Mar 2011

Iqbal Tahir, Idrees Muhammad, Ali Liaqat, Hussain Abrar, Ali Muhammad, Butt Sadia, Yousaf Muhammad Zubair, Sabar Muhammad Farooq

Abstract excerpt

BACKGROUND: Pakistan is a highly endemic area for hepatitis E virus (HEV) infection. The aim of the current study was to isolate and characterize strains of HEV in two mini outbreaks. RESULTS: RNA was extracted and reverse transcribed to cDNA. Nested PCR was done for the detection of HEV RNA. The positive bands were eluted, cloned in TA vector and sequenced in both directions using genetic Analyzer (Applied...

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Identifiers

PMID: 21371336
DOI: 10.1186/1743-422X-8-94
PMCID: PMC3056816

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