Article
Labeling of multiple HIV-1 proteins with the biarsenical-tetracysteine system.
PloS one - 11 Feb 2011
Pereira Cândida F, Ellenberg Paula C, Jones Kate L, Fernandez Tara L, Smyth Redmond P, Hawkes David J, Hijnen Marcel, Vivet-Boudou Valérie, Marquet Roland, Johnson Iain, Mak Johnson
Abstract excerpt
Due to its small size and versatility, the biarsenical-tetracysteine system is an attractive way to label viral proteins for live cell imaging. This study describes the genetic labeling of the human immunodeficiency virus type 1 (HIV-1) structural proteins (matrix, capsid and nucleocapsid), enzymes (protease, reverse transcriptase, RNAse H and integrase) and envelope glycoprotein 120 with a tetracysteine tag in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
