Article
In vitro invasion efficiency and intracellular proliferation rate comprise virulence-related phenotypic traits of Neospora caninum.
Veterinary research - 23 Feb 2011
Regidor-Cerrillo Javier, Gómez-Bautista Mercedes, Sodupe Itsaso, Aduriz Gorka, Álvarez-García Gema, Del Pozo Itziar, Ortega-Mora Luis Miguel
Abstract excerpt
In this study, we examined the in vitro invasion and proliferation capacities of the Nc-Liv and ten Spanish Neospora caninum isolates (Nc-Spain 1 H - Nc-Spain 10). The invasion rate was determined as the number of tachyzoites that completed their internalisation into MARC-145 cells at 2, 4, and 6 h post-inoculation (pi). The proliferation rate was evaluated by determining the doubling time during the exponential...
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