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Haploid and doubled haploid plants from developing male and female gametes of Gentiana triflora.

Plant cell reports - 1 Jun 2011

Pathirana Ranjith, Frew Tonya, Hedderley Duncan, Timmerman-Vaughan Gail, Morgan Ed

Abstract excerpt

Protocols were developed for the generation of haploid or doubled haploid plants from developing microspores and ovules of Gentiana triflora. Plant regeneration was achieved using flower buds harvested at the mid to late uninucleate stages of microspore development and then treated at 4°C for 48 h prior to culture. Anthers and ovaries were cultured on modified Nitsch and Nitsch medium supplemented with a...

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PMID: 21293862
DOI: 10.1007/s00299-011-1012-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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