Article
Microsatellite standardization and evaluation of genotyping error in a large multi-partner research programme for conservation of Atlantic salmon (Salmo salar L.).
Genetica - 1 Mar 2011
Ellis J S, Gilbey J, Armstrong A, Balstad T, Cauwelier E, Cherbonnel C, Consuegra S, Coughlan J, Cross T F, Crozier W, Dillane E, Ensing D, García de Leániz C, García-Vázquez E, Griffiths A M, Hindar K, Hjorleifsdottir S, Knox D, Machado-Schiaffino G, McGinnity P, Meldrup D, Nielsen E E, Olafsson K, Primmer C R, Prodohl P, Stradmeyer L, Vähä J-P, Verspoor E, Wennevik V, Stevens J R
Abstract excerpt
Microsatellite genotyping is a common DNA characterization technique in population, ecological and evolutionary genetics research. Since different alleles are sized relative to internal size-standards, different laboratories must calibrate and standardize allelic designations when exchanging data. This interchange of microsatellite data can often prove problematic. Here, 16 microsatellite loci were calibrated and...
Topics
- Alleles
- Animals
- Conservation of Natural Resources
- Genetic Drift
- Genetic Variation
- Genotype
- Microsatellite Repeats
- Molecular Typing