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Detection of Staphylococcus hyicus exfoliative toxin genes by dot blot hybridization and multiplex polymerase chain reaction.

Microbiology and immunology - 1 Mar 2011

Onuma Kenta, Uoya Yusuke, Koide Tetsuo, Shibata Ayumi, Tanabe Taishi, Sato Hisaaki

Abstract excerpt

We designed a novel DNA probe and novel PCR primer sets for detecting the genes coding for Staphylococcus hyicus (S. hyicus) exfoliative toxin (ET). In dot blot hybridization, the novel DNA probe hybridized with chromosomal DNA of ExhA-, ExhB-, ExhC-, ExhD-, and SHETA-producing strains. This probe also hybridized with the plasmid DNA of a SHETB-producing strain. In Southern blot hybridization, the probe...

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PMID: 21244467
DOI: 10.1111/j.1348-0421.2011.00308.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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