Article
Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene.
Journal of clinical microbiology - 1 Sept 1998
Becker K, Roth R, Peters G
Abstract excerpt
Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search fo...
Topics
- Bacterial Toxins
- DNA Primers
- Electrophoresis, Agar Gel
- Enterotoxins
- Exfoliatins
- Humans
- Immunoenzyme Techniques
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Phenotype
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Staphylococcus aureus
- Superantigens