A rapid method to screen putative mRNA targets of any known microRNA

Abstract excerpt

BACKGROUND: microRNAs (miRNAs) are a group of regulatory RNAs that regulate gene expression by binding to specific sequences on target mRNAs. However, functional identification of mRNA targets is usually difficult and time consuming. Here we report hybrid-PCR as a new and rapid approach to screen putative mRNA targets in vitro. RESULTS: Fifteen putative target mRNAs for human cytomegalovirus (HCMV) miR-UL112-1,...

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