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[Genetic analysis of hantaviruses carried by Rattus norvegicus collected from Henan and Neimenggu provinces, China].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Sept 2010

Wang Wen, Lin Xian-dan, Guo Wen-ping, Li Ming-hui, Zhang Yong-zhen

Abstract excerpt

OBJECTIVE: Genetic analysis was performed to infer the relationship between hantaviruses carried by Rattus norvegicus from Henan and Neimenggu provinces and the other known hantavirus and the vaccine strain. METHODS: Total RNA was extracted from lung tissues with Trizol reagent. The complete M and S segment sequences of strains NM133 and Q12 were amplified by RT-PCR. The purified DNA fragments were directly...

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PMID: 21162871

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