Article
[Genotyping and sequence analysis on G2 segment of hantavirus carried by rat in Hebei province].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Jun 2009
Huang Xiao-Xia, Li Qi, Han Zhan-Ying, Zhang Yan-Bo, Wei Ya-Mei, Xu Yong-Gang
Abstract excerpt
OBJECTIVE: To know the genotype and subtype of hantavirus (HV) carried by host animals in HFRS natural epidemic area of Hebei province. METHODS: According to the conservative sequence of 76-118 and R22 strains, specific primers were designed. RT-nested PCR was used to amplify partial M segments from the positive rat lungs screened by IFA. Agarose gel electrophoresis was used to identify the types. Nucleotides...
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