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Article

Poly(T) variation in heteroderid nematode mitochondrial genomes is predominantly an artefact of amplification.

Journal of molecular evolution - 1 Feb 2011

Riepsamen Angelique H, Gibson Tracey, Rowe Janet, Chitwood David J, Subbotin Sergei A, Dowton Mark

Abstract excerpt

We assessed the rate of in vitro polymerase errors at polythymidine [poly(T)] tracts in the mitochondrial DNA (mtDNA) of a heteroderid nematode (Heterodera cajani). The mtDNA of these nematodes contain unusually high numbers of poly(T) tracts, and have previously been suggested to contain biological poly(T) length variation. However, using a cloned molecule, we observed that poly(T) variation was generated in...

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PMID: 21161202
DOI: 10.1007/s00239-010-9414-3

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