Article
Rapid discrimination and quantification of Theileria orientalis types using ribosomal DNA internal transcribed spacers.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Mar 2011
Kamau Joseph, Salim Bashir, Yokoyama Naoki, Kinyanjui Peter, Sugimoto Chihiro
Abstract excerpt
We report the population structure analysis of Theileria orientalis types (Ikeda, Buffeli and Chitose), the causative agent of theileriosis in cattle and its cohorts, using ITS1 and ITS2 spacers by fragment genotyping. We utilized primers flanking the two ribosomal RNA internal transcribed spacers (ITS1 and ITS2). Due to varying degrees of sequence polymorphism in the ITS regions found within and between species,...
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