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Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene

1 Dec 2010

Abstract excerpt

A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 x 10² DNA copies/μL, and the assay was linear in the range of 1 x 10² to 1 x 10⁹ copies/μL. There was no cross-reaction with porcine circovirus 2 (PCV2), porcine reproductive and...

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PMID: 21126330
DOI: 10.1186/1743-422x-7-353

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