Article
Effect of Saccharomyces cerevisiae ret1-1 mutation on glycosylation and localization of the secretome.
Molecules and cells - 1 Feb 2011
Kim Ki-Hyun, Kim Eun-Kyung, Kim Su-Jin, Park Yun-Hee, Park Hee-Moon
Abstract excerpt
To study the effect of the ret1-1 mutation on the secretome, the glycosylation patterns and locations of the secretory proteins and glycosyltransferases responsible for glycosylation were investigated. Analyses of secretory proteins and cell wall-associated glycoproteins showed severe impairment of glycosylation in this mutant. Results from 2D-polyacrylamide gel electrophoresis (PAGE) indicated defects in the...
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