Article
Replacement of isoleucine-397 by threonine in the clotting proteinase factor IXa (Los Angeles and Long Beach variants) affects macromolecular catalysis but not L-tosylarginine methyl ester hydrolysis. Lack of correlation between the ox brain prothrombin time and the mutation site in the variant proteins.
The Biochemical journal - 1 Jan 1990
Spitzer S G, Warn-Cramer B J, Kasper C K, Bajaj S P
Abstract excerpt
Previously, from the plasma of unrelated haemophilia-B patients, we isolated two non-functional Factor IX variants, namely Los Angeles (IXLA) and Long Beach (IXLB). Both variants could be cleaved to yield Factor IXa-like molecules, but were defective in catalysing the cleavage of Factor X (macrom...
Topics
- Animals
- Antigens
- Arginine
- Base Sequence
- Brain
- Catalysis
- Cattle
- DNA
- Esterases
- Factor IX
- Factor IXa
- Humans
- Hydrolysis
- Isoleucine
- Male