Article
Identification and characterization of a gene and protein required for glycosylation in the yeast Golgi.
Molecular microbiology - 1 Nov 1990
Devlin C, Ballou C E
Abstract excerpt
The MNN2 gene of Saccharomyces cerevisiae has been cloned by complementation of the mnn2 mutant phenotype scored by a change in cell surface carbohydrate structure resulting from a lack of alpha 1----2-mannose branching in the outer chain. The gene was subcloned as a 3 kb DNA fragment that integr...
Topics
- Cloning, Molecular
- DNA, Fungal
- Escherichia coli
- Fungal Proteins
- Genes, Fungal
- Genetic Complementation Test
- Genotype
- Glycosylation
- Golgi Apparatus
- Membrane Glycoproteins
- Nucleic Acid Hybridization
- Plasmids
- Protein Processing, Post-Translational
- Saccharomyces cerevisiae
- Spheroplasts