Article
Identification and characterization of a gene and protein required for glycosylation in the yeast Golgi.
Molecular microbiology - 1 Nov 1990
Devlin C, Ballou C E
Abstract excerpt
The MNN2 gene of Saccharomyces cerevisiae has been cloned by complementation of the mnn2 mutant phenotype scored by a change in cell surface carbohydrate structure resulting from a lack of alpha 1----2-mannose branching in the outer chain. The gene was subcloned as a 3 kb DNA fragment that integrated at the MNN2 locus, and a gene disruption yielded the mnn2 phenotype. A lacZ-MNN2 gene fusion protein, produced in...
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