Article
Allelic diversity at MHC class II DQ loci in buffalo (Bubalus bubalis): evidence for duplication.
Veterinary immunology and immunopathology - 1 Dec 2010
Niranjan Saket K, Deb Sitangsu M, Kumar Subodh, Mitra Abhijit, Sharma Arjava, Sakaram Durgam, Naskar Soumen, Sharma Deepak, Sharma Sita R
Abstract excerpt
The genetic diversity of MHC class II DQ genes was investigated in riverine buffalo (Bubalus bubalis) by PCR-RFLP and sequencing. Highly variable regions (exons 2-3) of DQ genes were amplified from 152 buffaloes and genotyped by PCR-RFLP. Alleles identified by differential restriction patterns were sequenced for the characterization. PCR-RFLP was a rapid method to discriminate between DQA1 and duplicated DQA2...
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