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Evaluating levels of PCR efficiency and genotyping error in DNA extracted from engorged and non-engorged female Dermacentor variabilis ticks.

Medical and veterinary entomology - 1 Mar 2011

Dharmarajan G, Rhodes O E

Abstract excerpt

Polymerase chain reaction (PCR)-based methods are increasingly used to elucidate tick biology. However, DNA extracted from ticks may provide poor PCR templates as a result of PCR inhibition by mammalian blood or contamination by male DNA (in fertilized females). In this study, the effects of removing the bloodmeal and reproductive organs were evaluated through paired DNA extractions in engorged and non-engorged...

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PMID: 20704654
DOI: 10.1111/j.1365-2915.2010.00903.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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