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Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus).

Molecular ecology - 1 Jul 2001

Morin P A, Chambers K E, Boesch C, Vigilant L

Abstract excerpt

Noninvasive samples are useful for molecular genetic analyses of wild animal populations. However, the low DNA content of such samples makes DNA amplification difficult, and there is the potential for erroneous results when one of two alleles at heterozygous microsatellite loci fails to be amplified. In this study we describe an assay designed to measure the amount of amplifiable nuclear DNA in low DNA...

Topics

Africa
Animals
DNA
Feces
Genotype
Hair
Humans
Microsatellite Repeats
Pan troglodytes
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 11472550
DOI: 10.1046/j.0962-1083.2001.01308.x

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