Article
Establishment of quantitative sequencing and filter contact vial bioassay for monitoring pyrethroid resistance in the common bed bug, Cimex lectularius.
Journal of medical entomology - 1 Jul 2010
Seong Keon Mook, Lee Da-Young, Yoon Kyong Sup, Kwon Deok Ho, Kim Heung Chul, Klein Terry A, Clark J Marshall, Lee Si Hyeock
Abstract excerpt
Two point mutations (V419L and L925I) in the voltage-sensitive sodium channel alpha-subunit gene have been identified in deltamethrin-resistant bed bugs. A quantitative sequencing (QS) protocol was developed to establish a population-based genotyping method as a molecular resistance-monitoring tool based on the frequency of the two mutations. The nucleotide signal ratio at each mutation site was generated from...
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