Article
Mutation of high-affinity methionine permease contributes to selenomethionyl protein production in Saccharomyces cerevisiae.
Applied and environmental microbiology - 1 Oct 2010
Kitajima Toshihiko, Chiba Yasunori, Jigami Yoshifumi
Abstract excerpt
The production of selenomethionine (SeMet) derivatives of recombinant proteins allows phase determination by single-wavelength or multiwavelength anomalous dispersion phasing in X-ray crystallography, and this popular approach has permitted the crystal structures of numerous proteins to be determined. Although yeast is an ideal host for the production of large amounts of eukaryotic proteins that require...
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